genecloning相关论文
Trehalose synthase is an important functional enzyme in the synthesis of trehalose in organisms and also participates in......
Cloning of catalase gene and antioxidant genes in Scophthalmus maximus response to metalloprotease o
Metalloproteases represent a class of extracellular proteases found in Vibrio anguillarum that can generate toxic and pa......
Locus TUTOU2 determines the panicle apical abortion phenotype of rice (Oryza sativa L.) in tutou2 mu
Rice panicle apical abortion (PAA) is a detrimental agronomic trait resulting in spikelet number reduction and yield los......
Molecular Cloning,Characterization and Expression Profile of Myf5 and Myf6 During Growth and Develop
Myogenic Regulatory Factors (MRFs) is involved in the muscle growth and differentiation.In this study,the cDNA sequence ......
With four specific primers of lipopetide antibiotics synthesis related genes, the Bacillus subtilis Czk1 genome genetic ......
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Cloning of genes encoding allergenic proteins and salt tolerance by screening a full-length cDNA lib
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Functional characterization of the apple putative UDP-glucose 4-epimerase 2 gene, MdUGE2: a potentia
In apple peel,there are two synthesis pathways of UDP-galactose.One is UDP-glucose pathway catalyzed by UDP-glucose 4-ep......
[Objective ]The objectives of this study are to clone goat PHLDA2(pleckstrin homology-like domain,family A,member 2),to ......
A full-length cDNA sequence coding calcium-dependent protein kinase (CDPK) of mulberry tree,which we designated MCDPK,wa......
Molecular Cloning, Sequence Analysis, and Expression of the Methionine sulfoxide reductase Gene (MSR
A full-length cDNA sequence encoding methionine sulfoxide reductase (MSR) from mulberry,which we designated MMSR was clo......
For the sake of keeping the balance of plant cells,Calcium,as a necessary nutrition element to plant growth development,......
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‘华特’猕猴桃是从野生毛花猕猴桃中选育出的新品种,平均单果质量82.00 ～94.37 g,是野生种的2～4倍,最大132.2 g.可溶性固形物含量14......
A MAPKK kinase gene was firstly cloned from Dunaliela salina by RT-PCR and RACE technology.The full length of cDNA for M......
With the approach of homologous cloning,the conserved fragment of cinnamyl alcohol dehydrogenase(CAD)was obtained throug......
Gene Cloning, Expression and Reducing Property Enhancement of Nitrous oxide reductase from Alcaligen
Nitrous oxide (N2O) is a small but potent greenhouse gas and tends to accumulate as an intermediate in the process of ba......
Molecular Cloning and Characterization of ATP-binding Cassette Transporters from the Bird Cherry Oat
Rhopalosiphum padi is an important pest of wheat,which is widely distributed throughout the world,causing heavy economic......
Knottedl-like homeobox (KNOX) proteins, homeodomain transcription factors, are characteristically expressed in shoot api......
Dahurian Larch, Larix gmelinii Rupr, one of the three major timber coniferous in northeast China, has straight timber, c......
Cloning, expression and functional analysis of mamZ gene participating in magnetosome biosynthesis o
For seeking genes associating with magnetosome biosynthesis mechanism in magnetotactic bacteria cells,a magnetosome-dele......
Cloning and characterization of three novel manganese peroxidase genes from the ligninolytic basidio
In this study, three full-length manganese peroxidase (MnP) genes from white-rot fungus Trametes gibbosa, termed Tgmnp1,......
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MOLECULAR CLONING, CHARACTERIZATION AND EXPRESSION OF THE RSR1 FROM YEAST FORM PENICILLIUM MARNEFFEI
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1 Introduction Dunaliella Salina,which taxi Dunaliella,Volvocales,Chlorophyceae Chlorophyta,is unicell algae with double......
A full-length cDNA sequence coding a primary amine oxidase 2(PAO2)of mulberry tree,which we designated MmPAO2,was cl......
1-deoxy-5-phosphate synthase(DXS)is a key enzyme in the upstream of terpene biosynthesis pathway,we cloned its gene ......
Isolation, expression, and promoter analysis of FmWRKY7 and 45Two novel transcription factor genes f
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Cloning and functional research of bacterial blight resistance candidate genes of Oryza granulata in
Bacterial blight(BB)is one of the main bacterial diseases in rice.In view of the narrow resistance spectrum of resistanc......
Ectopic overexpression of maize (Zea mays L.) ZmEBP1 gene enhanced organ size in Arabidopsis thalian
High-yield crops are the main goal that crop breeders have been striving for.As an important trait, organ size determine......
Cloning and characterization of three novel low molecular weight glutenin subunit genes from Triticu
Two novel low-molecular-weight glutenin subunit (LMW-GS) genes, designated TatLMW-i1, TatLMW-ic1and TatLMW-ic2, were clo......
Functional genomics requires vector construction for protein expression and functional characterization of target genes;......
Cloning and characterization of a novel manganese peroxidase gene from the ligninolytic basidiomycet
A cDNA and genomic sequence encoding manganese peroxidase from T.gibbosa were cloned and sequenced.The cDNA, encodes......
TRIM5α,encoded by TRIM5 gene belonging to TRIM superfamily,is the most appealing star in resisting retrovirus infe......
Cloning and functional characterization of a wheat serinethreonine kinase gene (TaSTK) related to sa
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从日本晴背景突变体库中筛选出一个小圆粒突变体small grain 4（sg4），通过一系列BR敏感性测试，发现其表达具有组织特异性．但与传统的BR缺......
产生抗冻蛋白（antifreeze protein,AFP）是许多昆虫抵御寒冷的一种重要机制。昆虫抗冻蛋白基因的克隆和表达是研究抗冻蛋白活性和功能......
目的构建DOC-1R（deleted in oral cancer-1related）的原核表达载体并且纯化其重组蛋白，用于进-步研究DOC-1R蛋白的结构与功能。方法采......
利用381A型DNA合成仪,分29个寡聚核苷酸片段化学合成了小鼠IL-4全基因,共442bp。以pUC12质粒作为载体,将所有合成片段分前后两组进......
将编码人单核细胞趋化蛋白－１（ＭＣＰ－１）的基因亚克隆到大肠杆菌表达载体ｐＥＸ３１Ａ中，在大肠杆菌中表达出ＭＳ２／ＭＣＰ－１融合蛋白，该表达产物约占菌体总蛋白的１５％左右，Ｗｅｓｔｅｒｎ ｂｌｏｔ检测表......
作为导向药物蓖麻毒素Ａ链在大肠杆菌中表达时不含糖基侧链，在体内半衰期长，可提高共作为导向药物的疗效。我们根据Ｒｉｃｉｎ基因核苷酸序列，设计......
用聚合酶链反应（ＰＣＲ）以大肠杆菌ＪＭ８３基因组ＤＮＡ为模板，扩增了精氨酰ｔ－ＲＮＡ合成酶基因，将该基因重组到载体ｐＵＣ１８上转化到大肠杆菌ＴＧ１中，得到在转化子中ＡｒｇＲＳ的高表达。精......
The Human Genome Project was launched at the end of the 1980s.Since then,the cloning and identification of functional ge......
Molecular characterization and expression of the SiUCP2 gene in sea urchin Strongylocentrotus interm
Uncoupling protein 2 (UCP2) is a proton transporter located in the inner mitochondrial membrane, and inhibits the format......
To clone genes specifically expressed in the placenta of patients with preeclampsia, and to explain the mechanism in the......
Sequence analysis and functional study of the Han Nationality glial cell line-derived neurotrophic f
To study the sequence and function of the glial cell line-derived neurotrophic factor (GDNF) transcript in subjects of H......
目的 从亚洲带绦虫成虫cDNA质粒文库中识别并预测自吞噬相关蛋白(autophagy related protein3-like,APG3)基因,并将该基因进行克隆......
